p60TRP mouse models

Our transgenic neuronal p60TRP bioluminescence mouse model allows in vivo analysis in living animals.

tgp60trp

Our transgenic p60TRP mice allow in vivo analysis in living mice to study memory & learning effects, as well as higher brain cognitive functions

Neuron-specific over-expression of p60TRP & luciferase in the mouse brain allows us to investigate in vivo in living mice the effect of p60TRP on brain development and cognitive functions in a spatial and temporal dependent manner.

p60TRP brain imaging

 

tg-p60trp enahnced cog funct

P60TRP improves cognitive functions in mice. Results obtained from twoday RAWM testing in p60TRP transgenic mice as described in supplemental ‘Materials and methods’. Data were collected from three-months-old p60TRP transgenic mice and wild-type littermates. Each block consists of four trials. Average error committed by p60TRP transgenic mice for each trial block. The data shown were obtained from female p60TRP transgenic mouse line-1 (S1 38) (diamond, n 8) and wild-type littermates (squares, n 8) (A). Average error of male mice for each trial block. The data shown were obtained from p60TRP transgenic mouse line-1 (S1) (diamond, n 10) and wildtype littermates (squares, n 10) (B). Average error of male and female mice for each trial block. The data shown were obtained from p60TRP transgenic mouse line-1 (S1) (diamond, n 12) and wildtype littermates (squares, n 12) (C). Average error of male and female mice for each trial block. The data shown were obtained from the p60TRP transgenic mouse line-2 (S2 31) (diamond, n 12) and wild-type littermates (squares, n 12). The data shown in A-F were collected independently on separate days with new mice each time. Data are presented as the mean  SD (by ANOVA) (*P 0.05 compared with controls) (D)–(F).

tg-p60trp mice conclusions

 

as well as general systems biology studies:

tg-p60TRP-heart

Neuronal over-expression of p60TRP affects various cardiac protein functions. Red color: activation of Nnt-, Txnl1/Rab5-, Ang/MasR/Rab5/Col6a3-signaling pathways; green color: inhibition of Ckb/m-, Atp5- and Ak1-signaling pathways; which in turn modulate pivotal cardiac myocytes’ K+ channels’ activities and cardiac output.

 

for our knock-out p6oTRP mice, please refer to the review by Heese et al, 2013.

References:

Brain-site-specific proteome changes induced by neuronal P60TRP expression.
Manavalan A, Mishra M, Sze SK, Heese K.
Neurosignals. 2013;21(3-4):129-49.

G proteins, p60TRP, and neurodegenerative diseases.
Heese K.
Mol Neurobiol. 2013 Jun;47(3):1103-11. (Review)

Neuronal p60TRP expression modulates cardiac capacity.
Mishra M, Manavalan A, Sze SK, Heese K.
J Proteomics. 2012 Feb 16;75(5):1600-17.

P60TRP interferes with the GPCR/secretase pathway to mediate neuronal survival and synaptogenesis.
Mishra M, Heese K.
J Cell Mol Med. 2011 Nov;15(11):2462-77.

 

Posted in Research, Systems Biology